Organization of DNA Partners and Strand Exchange Mechanisms during Flp Site-Specific Recombination Analyzed by Difference Topology, Single Molecule FRET and Single Molecule TPM

Chien Hui; Yen Ting Liu; Christos G. Savva; Paul A. Rowley; Brian Cannon; Hsiu Fang Fan; Rick Russell; Andreas Holzenburg; Makkuni Jayaram

doi:10.1016/j.jmb.2013.11.017

Organization of DNA Partners and Strand Exchange Mechanisms during Flp Site-Specific Recombination Analyzed by Difference Topology, Single Molecule FRET and Single Molecule TPM

Chien Hui, Yen Ting Liu, Christos G. Savva, Paul A. Rowley, Brian Cannon, Hsiu Fang Fan, Rick Russell, Andreas Holzenburg, Makkuni Jayaram

Research output: Contribution to journal › Article › peer-review

Original language	American English
Journal	Journal of Molecular Biology
Volume	426
Issue number	4
DOIs	https://doi.org/10.1016/j.jmb.2013.11.017
State	Published - Jan 2 2014
Externally published	Yes

Disciplines

Molecular Biology
Genetics

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10.1016/j.jmb.2013.11.017License: Unspecified

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Hui, C., Liu, Y. T., Savva, C. G., Rowley, P. A., Cannon, B., Fan, H. F., Russell, R., Holzenburg, A., & Jayaram, M. (2014). Organization of DNA Partners and Strand Exchange Mechanisms during Flp Site-Specific Recombination Analyzed by Difference Topology, Single Molecule FRET and Single Molecule TPM. Journal of Molecular Biology, 426(4). https://doi.org/10.1016/j.jmb.2013.11.017

Hui, C, Liu, YT, Savva, CG, Rowley, PA, Cannon, B, Fan, HF, Russell, R, Holzenburg, A & Jayaram, M 2014, 'Organization of DNA Partners and Strand Exchange Mechanisms during Flp Site-Specific Recombination Analyzed by Difference Topology, Single Molecule FRET and Single Molecule TPM', Journal of Molecular Biology, vol. 426, no. 4. https://doi.org/10.1016/j.jmb.2013.11.017

@article{f49b48e55cd841e9811f0d9378925d35,

title = "Organization of DNA Partners and Strand Exchange Mechanisms during Flp Site-Specific Recombination Analyzed by Difference Topology, Single Molecule FRET and Single Molecule TPM",

author = "Chien Hui and Liu, \{Yen Ting\} and Savva, \{Christos G.\} and Rowley, \{Paul A.\} and Brian Cannon and Fan, \{Hsiu Fang\} and Rick Russell and Andreas Holzenburg and Makkuni Jayaram",

note = "Flp site-specific recombination between two target sites ( FRTs) harboring non-homology within the strand exchange region does not yield stable recombinant products. In negatively supercoiled plasmids containing head-to-tail sites, the reaction produces a series of knots with odd-numbered crossings. When the sites are in head-to-head orientation, the knot products contain even-numbered crossings.",

year = "2014",

month = jan,

day = "2",

doi = "10.1016/j.jmb.2013.11.017",

language = "American English",

volume = "426",

journal = "Journal of Molecular Biology",

number = "4",

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TY - JOUR

T1 - Organization of DNA Partners and Strand Exchange Mechanisms during Flp Site-Specific Recombination Analyzed by Difference Topology, Single Molecule FRET and Single Molecule TPM

AU - Hui, Chien

AU - Liu, Yen Ting

AU - Savva, Christos G.

AU - Rowley, Paul A.

AU - Cannon, Brian

AU - Fan, Hsiu Fang

AU - Russell, Rick

AU - Holzenburg, Andreas

AU - Jayaram, Makkuni

N1 - Flp site-specific recombination between two target sites ( FRTs) harboring non-homology within the strand exchange region does not yield stable recombinant products. In negatively supercoiled plasmids containing head-to-tail sites, the reaction produces a series of knots with odd-numbered crossings. When the sites are in head-to-head orientation, the knot products contain even-numbered crossings.

PY - 2014/1/2

Y1 - 2014/1/2

UR - https://www.sciencedirect.com/science/article/pii/S0022283613007286

U2 - 10.1016/j.jmb.2013.11.017

DO - 10.1016/j.jmb.2013.11.017

M3 - Article

VL - 426

JO - Journal of Molecular Biology

JF - Journal of Molecular Biology

IS - 4

ER -

Organization of DNA Partners and Strand Exchange Mechanisms during Flp Site-Specific Recombination Analyzed by Difference Topology, Single Molecule FRET and Single Molecule TPM

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