Improving suction technology for nerve activity recording

Extracellular recording of nerve activities using suction electrodes is an easy yet powerful tool in characterizing neural activities in physiology and pathological conditions. The key factors that determine the quality of suction electrode recordings have not been fully investigated. New Methods: Here, we proposed a biophysical model to study the mechanisms underlying suction technology for axon recording. The model focuses on the interpretation of the recorded single neuron activity based on the location of the electrode, the integrity of the recorded tissue, and the tightness of the suction. To directly test these model predictions, we applied two channel recordings from the nerves in Aplysia californica, and analyzed the shape of the extracellularly recorded single neuron activity under various conditions.