TY - JOUR
T1 - Effect of the double mutation Y39A/R75A on the allosteric regulation of ADP-glucose pyrophosphorylase from agrobacterium tumefaciens
AU - Bennett, Thomas
AU - Martinez-Ramirez, Gabriela
AU - Alghamdi, Mashael
AU - Hussien, Raina
AU - Liu, Dali
AU - Ballicora, Miguel A.
PY - 1800
Y1 - 1800
N2 - ADP-glucose pyrophosphorylase is the enzyme responsible for the production of ADP-glucose, which is used for the synthesis of starch in plants and glycogen in bacteria. This enzyme within our model organism Agrobacterium tumefaciens is allosterically regulated through various activators and inhibitors. Previously single mutation residues at predetermined sites of interest within the wild type of A. tumefaciens have been studied. Two of these sites Tyr 39 and Arg 75, are residues located on the allosteric binding site of the activator Fru6P. Therefore, we targeted this site with a double mutation of both residues to an alanine and observed the results.
AB - ADP-glucose pyrophosphorylase is the enzyme responsible for the production of ADP-glucose, which is used for the synthesis of starch in plants and glycogen in bacteria. This enzyme within our model organism Agrobacterium tumefaciens is allosterically regulated through various activators and inhibitors. Previously single mutation residues at predetermined sites of interest within the wild type of A. tumefaciens have been studied. Two of these sites Tyr 39 and Arg 75, are residues located on the allosteric binding site of the activator Fru6P. Therefore, we targeted this site with a double mutation of both residues to an alanine and observed the results.
UR - https://ecommons.luc.edu/ures/2022/2022/367
M3 - Article
JO - Undergraduate Research and Engagement Symposium
JF - Undergraduate Research and Engagement Symposium
ER -