TY - JOUR
T1 - Culturing of Female Bladder Bacteria Reveals an Interconnected Urogenital Microbiota
AU - Thomas-White, Krystal
AU - Forster, Samuel C.
AU - Kumar, Nitin
AU - Van Kuiken, Michelle
AU - Putonti, Catherine
AU - Stares, Mark D.
AU - Hilt, Evann E.
AU - Price, Travis K.
AU - Wolfe, Alan J.
AU - Lawley, Trevor D.
PY - 2018/4/19
Y1 - 2018/4/19
N2 - Metagenomic analyses have indicated that the female bladder harbors an indigenous microbiota. However, there are few cultured reference strains with sequenced genomes available for functional and experimental analyses. Here we isolate and genome-sequence 149 bacterial strains from catheterized urine of 77 women. This culture collection spans 78 species, representing approximately two thirds of the bacterial diversity within the sampled bladders, including Proteobacteria, Actinobacteria, and Firmicutes. Detailed genomic and functional comparison of the bladder microbiota to the gastrointestinal and vaginal microbiotas demonstrates similar vaginal and bladder microbiota, with functional capacities that are distinct from those observed in the gastrointestinal microbiota. Whole-genome phylogenetic analysis of bacterial strains isolated from the vagina and bladder in the same women identifies highly similar Escherichia coli, Streptococcus anginosus, Lactobacillus iners, and Lactobacillus crispatus, suggesting an interlinked female urogenital microbiota that is not only limited to pathogens but is also characteristic of health-associated commensals.
AB - Metagenomic analyses have indicated that the female bladder harbors an indigenous microbiota. However, there are few cultured reference strains with sequenced genomes available for functional and experimental analyses. Here we isolate and genome-sequence 149 bacterial strains from catheterized urine of 77 women. This culture collection spans 78 species, representing approximately two thirds of the bacterial diversity within the sampled bladders, including Proteobacteria, Actinobacteria, and Firmicutes. Detailed genomic and functional comparison of the bladder microbiota to the gastrointestinal and vaginal microbiotas demonstrates similar vaginal and bladder microbiota, with functional capacities that are distinct from those observed in the gastrointestinal microbiota. Whole-genome phylogenetic analysis of bacterial strains isolated from the vagina and bladder in the same women identifies highly similar Escherichia coli, Streptococcus anginosus, Lactobacillus iners, and Lactobacillus crispatus, suggesting an interlinked female urogenital microbiota that is not only limited to pathogens but is also characteristic of health-associated commensals.
KW - Metagenomic analysis
KW - indigenous microbiota
KW - female urogenital microbiota
KW - Metagenomic Analyses
KW - Female Bladder
KW - Microbiota
KW - Genomes
KW - Proteobacteria
KW - Actinobacteria
KW - Firmicutes
UR - https://ecommons.luc.edu/bioinformatics_facpub/49
UR - https://ecommons.luc.edu/biology_facpubs/92
U2 - 10.1038/s41467-018-03968-5
DO - 10.1038/s41467-018-03968-5
M3 - Article
VL - 9
JO - Bioinformatics Faculty Publications
JF - Bioinformatics Faculty Publications
ER -