Aerococcus urinae Isolated from Women with Lower Urinary Tract Symptoms: In Vitro Aggregation and Genome Analysis

Evann E Hilt; Catherine Putonti; Krystal Thomas-White; Amanda L Lewis; Karen L Visick; Nicole M Gilbert; Alan J Wolfe

doi:10.1128/JB.00170-20

Aerococcus urinae Isolated from Women with Lower Urinary Tract Symptoms: In Vitro Aggregation and Genome Analysis

Evann E Hilt, Catherine Putonti, Krystal Thomas-White, Amanda L Lewis, Karen L Visick, Nicole M Gilbert, Alan J Wolfe

Department of Biology

Research output: Contribution to journal › Article › peer-review

Abstract

Aerococcus urinae is increasingly recognized as a potentially significant urinary tract bacterium. A. urinae has been isolated from urine collected from both males and females with a wide range of clinical conditions, including urinary tract infection (UTI), urgency urinary incontinence (UUI), and overactive bladder (OAB). A. urinae is of particular clinical concern because it is highly resistant to many antibiotics and, when undiagnosed, can cause invasive and life-threatening bacteremia, sepsis, or soft tissue infections. Previous genomic characterization studies have examined A. urinae strains isolated from patients experiencing UTI episodes. Here, we analyzed the genomes of A. urinae strains isolated as part of the urinary microbiome from patients with UUI or OAB. Furthermore, we report that certain A. urinae strains exhibit aggregative in vitro phenotypes, including flocking, which can be modified by various growth medium conditions. Finally, we performed in-depth genomic comparisons to identify pathways that distinguish flocking and nonflocking strains. IMPORTANCE Aerococcus urinae is a urinary bacterium of emerging clinical interest. Here, we explored the ability of 24 strains of A. urinae isolated from women with lower urinary tract symptoms to display aggregation phenotypes in vitro . We sequenced and analyzed the genomes of these A. urinae strains. We performed functional genomic analyses to determine whether the in vitro hyperflocking aggregation phenotype displayed by certain A. urinae strains was related to the presence or absence of certain pathways. Our findings demonstrate that A. urinae strains have different propensities to display aggregative properties in vitro and suggest a potential association between phylogeny and flocking.

Original language	American English
Journal	Bioinformatics Faculty Publications
Volume	202
Issue number	13
DOIs	https://doi.org/10.1128/JB.00170-20
State	Published - Jun 9 2020

Keywords

Gram positive
aggregation
bladder
lower urinary tract symptoms
urinary microbiome

Disciplines

Bioinformatics

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@article{53d8dd041fbd4d1a93551836c6face7b,

title = "Aerococcus urinae Isolated from Women with Lower Urinary Tract Symptoms: In Vitro Aggregation and Genome Analysis",

abstract = " Aerococcus urinae is increasingly recognized as a potentially significant urinary tract bacterium. A. urinae has been isolated from urine collected from both males and females with a wide range of clinical conditions, including urinary tract infection (UTI), urgency urinary incontinence (UUI), and overactive bladder (OAB). A. urinae is of particular clinical concern because it is highly resistant to many antibiotics and, when undiagnosed, can cause invasive and life-threatening bacteremia, sepsis, or soft tissue infections. Previous genomic characterization studies have examined A. urinae strains isolated from patients experiencing UTI episodes. Here, we analyzed the genomes of A. urinae strains isolated as part of the urinary microbiome from patients with UUI or OAB. Furthermore, we report that certain A. urinae strains exhibit aggregative in vitro phenotypes, including flocking, which can be modified by various growth medium conditions. Finally, we performed in-depth genomic comparisons to identify pathways that distinguish flocking and nonflocking strains. IMPORTANCE Aerococcus urinae is a urinary bacterium of emerging clinical interest. Here, we explored the ability of 24 strains of A. urinae isolated from women with lower urinary tract symptoms to display aggregation phenotypes in vitro . We sequenced and analyzed the genomes of these A. urinae strains. We performed functional genomic analyses to determine whether the in vitro hyperflocking aggregation phenotype displayed by certain A. urinae strains was related to the presence or absence of certain pathways. Our findings demonstrate that A. urinae strains have different propensities to display aggregative properties in vitro and suggest a potential association between phylogeny and flocking. ",

keywords = "Gram positive, aggregation, bladder, lower urinary tract symptoms, urinary microbiome",

author = "Hilt, \{Evann E\} and Catherine Putonti and Krystal Thomas-White and Lewis, \{Amanda L\} and Visick, \{Karen L\} and Gilbert, \{Nicole M\} and Wolfe, \{Alan J\}",

note = "Hilt EE, Putonti C, Thomas-White K, Lewis AL, Visick KL, Gilbert NM, Wolfe AJ. 2020. Aerococcus urinae isolated from women with lower urinary tract symptoms: in vitro aggregation and genome analysis. J Bacteriol 202:e00170-20. https://doi.org/10.1128/JB.00170-20.",

year = "2020",

month = jun,

day = "9",

doi = "10.1128/JB.00170-20",

language = "American English",

volume = "202",

journal = "Bioinformatics Faculty Publications",

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T1 - Aerococcus urinae Isolated from Women with Lower Urinary Tract Symptoms: In Vitro Aggregation and Genome Analysis

AU - Hilt, Evann E

AU - Putonti, Catherine

AU - Thomas-White, Krystal

AU - Lewis, Amanda L

AU - Visick, Karen L

AU - Gilbert, Nicole M

AU - Wolfe, Alan J

N1 - Hilt EE, Putonti C, Thomas-White K, Lewis AL, Visick KL, Gilbert NM, Wolfe AJ. 2020. Aerococcus urinae isolated from women with lower urinary tract symptoms: in vitro aggregation and genome analysis. J Bacteriol 202:e00170-20. https://doi.org/10.1128/JB.00170-20.

PY - 2020/6/9

Y1 - 2020/6/9

N2 - Aerococcus urinae is increasingly recognized as a potentially significant urinary tract bacterium. A. urinae has been isolated from urine collected from both males and females with a wide range of clinical conditions, including urinary tract infection (UTI), urgency urinary incontinence (UUI), and overactive bladder (OAB). A. urinae is of particular clinical concern because it is highly resistant to many antibiotics and, when undiagnosed, can cause invasive and life-threatening bacteremia, sepsis, or soft tissue infections. Previous genomic characterization studies have examined A. urinae strains isolated from patients experiencing UTI episodes. Here, we analyzed the genomes of A. urinae strains isolated as part of the urinary microbiome from patients with UUI or OAB. Furthermore, we report that certain A. urinae strains exhibit aggregative in vitro phenotypes, including flocking, which can be modified by various growth medium conditions. Finally, we performed in-depth genomic comparisons to identify pathways that distinguish flocking and nonflocking strains. IMPORTANCE Aerococcus urinae is a urinary bacterium of emerging clinical interest. Here, we explored the ability of 24 strains of A. urinae isolated from women with lower urinary tract symptoms to display aggregation phenotypes in vitro . We sequenced and analyzed the genomes of these A. urinae strains. We performed functional genomic analyses to determine whether the in vitro hyperflocking aggregation phenotype displayed by certain A. urinae strains was related to the presence or absence of certain pathways. Our findings demonstrate that A. urinae strains have different propensities to display aggregative properties in vitro and suggest a potential association between phylogeny and flocking.

AB - Aerococcus urinae is increasingly recognized as a potentially significant urinary tract bacterium. A. urinae has been isolated from urine collected from both males and females with a wide range of clinical conditions, including urinary tract infection (UTI), urgency urinary incontinence (UUI), and overactive bladder (OAB). A. urinae is of particular clinical concern because it is highly resistant to many antibiotics and, when undiagnosed, can cause invasive and life-threatening bacteremia, sepsis, or soft tissue infections. Previous genomic characterization studies have examined A. urinae strains isolated from patients experiencing UTI episodes. Here, we analyzed the genomes of A. urinae strains isolated as part of the urinary microbiome from patients with UUI or OAB. Furthermore, we report that certain A. urinae strains exhibit aggregative in vitro phenotypes, including flocking, which can be modified by various growth medium conditions. Finally, we performed in-depth genomic comparisons to identify pathways that distinguish flocking and nonflocking strains. IMPORTANCE Aerococcus urinae is a urinary bacterium of emerging clinical interest. Here, we explored the ability of 24 strains of A. urinae isolated from women with lower urinary tract symptoms to display aggregation phenotypes in vitro . We sequenced and analyzed the genomes of these A. urinae strains. We performed functional genomic analyses to determine whether the in vitro hyperflocking aggregation phenotype displayed by certain A. urinae strains was related to the presence or absence of certain pathways. Our findings demonstrate that A. urinae strains have different propensities to display aggregative properties in vitro and suggest a potential association between phylogeny and flocking.

KW - Gram positive

KW - aggregation

KW - bladder

KW - lower urinary tract symptoms

KW - urinary microbiome

UR - https://ecommons.luc.edu/bioinformatics_facpub/94

UR - https://digitalcommons.wustl.edu/open_access_pubs/9176

U2 - 10.1128/JB.00170-20

DO - 10.1128/JB.00170-20

M3 - Article

VL - 202

JO - Bioinformatics Faculty Publications

JF - Bioinformatics Faculty Publications

IS - 13

ER -

Aerococcus urinae Isolated from Women with Lower Urinary Tract Symptoms: In Vitro Aggregation and Genome Analysis

Abstract

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