18S rDNA Dataset Profiling Microeukaryotic Populations Within Chicago Area Nearshore Waters

Daniel Searle; Emily Sible; Alexandria Cooper; Catherine Putonti

doi:10.1016/j.dib.2015.12.042

18S rDNA Dataset Profiling Microeukaryotic Populations Within Chicago Area Nearshore Waters

Daniel Searle, Emily Sible, Alexandria Cooper, Catherine Putonti

Department of Biology

Research output: Contribution to journal › Article › peer-review

Abstract

Despite their critical role in the aquatic food web and nutrient cycling, microeukaryotes within freshwater environments are under-studied. Herein we present the first high-throughput molecular survey of microeukaryotes within Lake Michigan. Every two weeks from May 13 to August 5, 2014, we collected surface water samples from the nearshore waters of four Chicago area beaches: Gillson Park, Montrose Beach, 57th Street Beach, and Calumet Beach. Four biological replicates were collected for each sampling date and location, resulting in 112 samples. Eighty-nine of these samples were surveyed through targeted sequencing of the V7 and V8 regions of the 18S rDNA gene. Both technical and biological replicates were sequenced and are included in this dataset. Raw sequence data is available via NCBI’s SRA database (BioProject PRJNA294919).

Original language	American English
Journal	Bioinformatics Faculty Publications
Volume	6
DOIs	https://doi.org/10.1016/j.dib.2015.12.042
State	Published - Jan 7 2016

Keywords

Microeukaryotic community
Metagenomics
Freshwater
Lake Michigan

Disciplines

Bioinformatics
Environmental Microbiology and Microbial Ecology
Terrestrial and Aquatic Ecology

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http://dx.doi.org/10.1016/j.dib.2015.12.042

Cite this

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title = "18S rDNA Dataset Profiling Microeukaryotic Populations Within Chicago Area Nearshore Waters",

abstract = " Despite their critical role in the aquatic food web and nutrient cycling, microeukaryotes within freshwater environments are under-studied. Herein we present the first high-throughput molecular survey of microeukaryotes within Lake Michigan. Every two weeks from May 13 to August 5, 2014, we collected surface water samples from the nearshore waters of four Chicago area beaches: Gillson Park, Montrose Beach, 57th Street Beach, and Calumet Beach. Four biological replicates were collected for each sampling date and location, resulting in 112 samples. Eighty-nine of these samples were surveyed through targeted sequencing of the V7 and V8 regions of the 18S rDNA gene. Both technical and biological replicates were sequenced and are included in this dataset. Raw sequence data is available via NCBI{\textquoteright}s SRA database (BioProject PRJNA294919).",

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author = "Daniel Searle and Emily Sible and Alexandria Cooper and Catherine Putonti",

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T1 - 18S rDNA Dataset Profiling Microeukaryotic Populations Within Chicago Area Nearshore Waters

AU - Searle, Daniel

AU - Sible, Emily

AU - Cooper, Alexandria

AU - Putonti, Catherine

PY - 2016/1/7

Y1 - 2016/1/7

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AB - Despite their critical role in the aquatic food web and nutrient cycling, microeukaryotes within freshwater environments are under-studied. Herein we present the first high-throughput molecular survey of microeukaryotes within Lake Michigan. Every two weeks from May 13 to August 5, 2014, we collected surface water samples from the nearshore waters of four Chicago area beaches: Gillson Park, Montrose Beach, 57th Street Beach, and Calumet Beach. Four biological replicates were collected for each sampling date and location, resulting in 112 samples. Eighty-nine of these samples were surveyed through targeted sequencing of the V7 and V8 regions of the 18S rDNA gene. Both technical and biological replicates were sequenced and are included in this dataset. Raw sequence data is available via NCBI’s SRA database (BioProject PRJNA294919).

KW - Microeukaryotic community

KW - Metagenomics

KW - Freshwater

KW - Lake Michigan

UR - https://ecommons.luc.edu/bioinformatics_facpub/26

U2 - 10.1016/j.dib.2015.12.042

DO - 10.1016/j.dib.2015.12.042

M3 - Article

VL - 6

JO - Bioinformatics Faculty Publications

JF - Bioinformatics Faculty Publications

ER -

18S rDNA Dataset Profiling Microeukaryotic Populations Within Chicago Area Nearshore Waters

Abstract

Keywords

Disciplines

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